Rotor-Gene® Q MDx
5plex HRM platform
Using an innovative rotary design, the Rotor-Gene Q MDx 5plex HRM Platform allows for unmatched thermal performance in real-time polymerase chain reaction (PCR) cycling. The use of a rotating disc ensures that every single tube is spun in a chamber of uniform temperature, using air as the conducting element. This allows for rapid thermal cycling and precise results in all tubes.
For detection, a set of six uncoupled excitation and emission filters are used in conjunction with six dedicated LEDs to maximize the detection capability. Each tube is illuminated by the excitation LED and the fluorescent signal transmitted to the PMT detector via a uniform optical path length. This eliminates well-to-well optical variation due to edge effect which block-based cycler had experienced. It also allows for fluorescent detection without the need of a reference dye.
With thermal and optical uniformity, the Rotor-Gene Q MDx 5plex HRM Platform offers unparalleled quantitative and genotyping analysis for PCR applications.
With five channels offering detection from the green to infrared wavelengths, the Rotor-Gene Q MDx 5plex Platform provides wide optics for most fluorophores available in the market. The decoupled filter sets create flexibility for customized excitation/detection wavelength combinations, making the platform compatible with most fluorophores.
| Channel | Excitation (nm) | Detection (nm) | Examples of fluorophores detected |
|---|---|---|---|
| Green | 470±10 | 510±5 | FAM, YYBR Green I, Fluorescein, EvaGreen, Alexa Fluor 488 |
| Yellow | 530±5 | 557±5 | JOE, VIC, HEX, TET, MAX, CAL Fluor Gold 540, Yakima Yellow |
| Orange | 585±5 | 610±5 | ROX, CAL Fluor Red 610, Cy 3.5, Texas Red, Alexa Fluor 568 |
| Red | 625±5 | 660±10 | Cy5, Quasar 670, LightCycler Red640, Alexa Fluor 663 |
| Crimson | 680±5 | 712 high pass | Quasar 705, LightCycler Red 705, Alexa Fluor 680 |
| High Resolution Melt (HRM) | 460±20 | 510±5 | SYBR Green I, SYTO 9, LC Green, LC Green Plus+, EvaGreen |
- Works in combination with Sentosa® SX101 for upstream sample extraction and PCR set-up
- Thermal non-uniformity of ±0.02°C
- Five sets of decoupled excitation/emission filters supporting a wide variety of dyes
- Optical uniformity allows PCR without the need of reference dye
- Rapid thermal heating and cooling using air as conducting element
- Optically stable LEDs with life time guarantee
- No optical calibration required during installation
- “Spin down” of tubes is not required
- Optical Temperature Verification (OTV) kit available for simple thermal verification for performance without the need for contacting a service technician
- Easy to clean, low maintenance & service requirement
- Small footprint (W37;H27.5;D42 cm) (W14.6;H10.8;D16.5 in)
| Product | Pack Size | Item Number |
|---|---|---|
| Rotor-Disc 72 Starter Kit (115V) and Accessories | 1 | 400047 |
| Rotor-Gene Q | 1 | 400046 |
